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. 2023 Jul 18;11(7):2019. doi: 10.3390/biomedicines11072019

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunocytochemistry and immunohistochemistry staining for HuR and the effect of niclosamide on HuR translocation. (a–d’) Immunofluorescence staining for the detection of HuR and the effect of niclosamide on HuR cytoplasmic translocation. WI-38 (a–b’) and NL20 (c–d’) cells were exposed to niclosamide or DMSO for 48 h. HuR is presented in green. Nuclei were counterstained with DAPI (blue). (e,e’) Immunohistochemistry staining for HuR protein in the LL/2 tumor tissue with (e) PBS or (e’) niclosamide treatment for 5 days. Arrows point to the cytoplasmic HuR.