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. 2023 Jul 13;13(7):1113. doi: 10.3390/biom13071113

Figure 7.

Figure 7

Evidence for the role of the myocardin-related transcription factor (MRTF)–serum response factor (SRF) pathway in the maturation of MG63 cells treated with Dcha-150 and FHBP. MG63s were co-stimulated with FHBP (500 nM) and Dcha-150 (Dc, 1 nM, as informed from Figure 5) for 72 h in the presence (“All”) and absence of CCG 203971 (CCG, 5 μM), an inhibitor of MRTF–SRF gene transcription. The clear and significant inhibition of ALP activity for cells exposed to all agents (** p < 0.001 compared to FHBP and Dc co-treated cells) supports the role of MRTF–SRF in the maturation of osteoblasts to FHBP–Dcha-150 co-stimulation. The data are the mean plus the standard deviation from two pooled, independent experiments, each performed on a different occasion using a different passage number of cells (n = 8 for Dc, n = 10 for all other groups).