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. 2023 Jul 1;13(7):1065. doi: 10.3390/biom13071065

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Topical 24,25 Vit D increases CD45+ cell numbers in the anterior stroma of diabetic mouse corneas. Mice were wounded with a 2 mm corneal epithelial debridement, and corneas were collected and immunostained with an anti-CD45 antibody (red) 8 h after corneal abrasion in (a) CTRL, (b) diabetic, (c) diabetic plus 1,25 Vit D and (d) diabetic plus 24,25-Vit D. (e) CD45+ numbers were quantified using the Imaris image processing software Spots package, demonstrating that 24,25 Vit D treatment elevated CD45+ immune cell numbers in wounded diabetic mouse corneas (n = 3, * p < 0.05).