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Effect of a mutation in either the strong or weak TyrR box on P2 repression
| lacZ fusion | β-Galactosidase sp act (U)a
|
|||
|---|---|---|---|---|
| tyrR366 | Multicopy tyrR+
|
|||
| MM | Phe | Tyr | ||
| P2wt-lacZ control | 110 | 20 (5.5) | 3 (37) | 3 (37) |
| P2wt-wb−-lacZ (weak box mutation) | 124 | 21 (6) | 7 (18) | 10 (13) |
| P2wt-sb−-lacZ (strong box mutation) | 114 | 78 (1.5) | 69 (1.7) | 66 (1.7) |
Designations and parenthetical values are as described in Table 2, footnote a.
