Table 2.
Plasmids used in this study.
| Name | Relevant characteristics | Source |
|---|---|---|
| pUDP002 | ori ampR panARS(OPT) AgTEF1p-hph-AgTEF1t ScTDH3p BsaI BsaI ScCYC1t AaTEF1p Spcas9-ScPHO5t | Gorter de Vries et al. 2017 |
| pUDPZb_ Gly_ADE2c_Asp | tRNAGly– ADE2 target—tRNAAsp cloned into pUDP002 | This study |
| pUDPZb_Gly_ADE2c_HDV | tRNAGly– ADE2 target—HDV cloned into pUDP002 | This study |
| pUDPZb_ HH_ADE2c_Gly | HH—ADE2 target—tRNAGly cloned into pUDP002 | This study |
| pUDPZb_ HH_ADE2c_HDV | HH—ADE2 target—HDV cloned into pUDP002 | This study |
| pSTBlue_repair chr1_kanMX | ori ampR panARS(OPT) US ADE2 AgTEF1p-kanMX-AgTEF1t DS ADE2 | This study |
| pSTBlue_repair chr2_NAT | ori ampR panARS(OPT) US ADE2 AgTEF1p-natMX-AgTEF1t DS ADE2 | This study |
| pZ3 | ori ampR panARS(OPT) AgTEF1p-kanMX-AgTEF1t | Branduardi et al. 2004 |
| pZ5 | ori ampR panARS(OPT) AgTEF1p-NAT-AgTEF1t | Branduardi et al. 2004 |
| pUDPZb_U1 | pUDP002 containing tRNAGly–BsaI restriction site– tRNAAsp | This study |
| pUDPZb_U1_DNL4 CT1 | DNL4 target1 inserted in the BsaI restriction site in pUDPZb_U1 | This study |
| PUDPZb_U1_DNL4 CT2 | DNL4 target2 inserted in the BsaI restriction site in pUDPZb_U1 | This study |
| PUDPZb_U1_DNL4 CT3 | DNL4 target3 inserted in the BsaI restriction site in pUDPZb_U1 | This study |
| PUDPZb_U1_DNL4 CT4 | DNL4 target4 inserted in the BsaI restriction site in pUDPZb_U1 | This study |
| PUDPZb_U1_DNL4 CT5 | DNL4 target5 inserted in the BsaI restriction site in pUDPZb_U1 | This study |