TABLE 3.
Genes of nitrogen metabolism and biosynthesis showing significant expression ratios
| Functional group | Gene | Gene Product | Log ratio (minimal/rich medium) |
|---|---|---|---|
| Amino acids | ilvC | Ketol-acid reductoisomerase | 0.977 |
| leuD | Isopropylmalate isomerase subunit | 0.951 | |
| tyrA | Chorismate mutase-T and prephenate dehydrogenase | 0.934 | |
| gltD | Glutamate synthase, small subunit | 0.889 | |
| aroF | 3-Deoxy-d-arabinoheptulosonate-7-phosphate synthase | 0.847 | |
| leuA | 2-Isopropylmalate synthase | 0.809 | |
| leuB | 3-Isopropylmalate dehydrogenase | 0.756 | |
| serA | d-3-Phosphoglycerate dehydrogenase | 0.633 | |
| ygjG | Probable ornithine aminotransferase | 0.623 | |
| leuC | 3-Isopropylmalate isomerase (dehydratase) subunit | 0.566 | |
| trpB | Tryptophan synthase, beta protein | 0.563 | |
| glyA | Serine hydroxymethyltransferase | 0.541 | |
| gdhA | NADP-specific glutamate dehydrogenase | 0.519 | |
| trpC | N-(5-Phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase | 0.518 | |
| ilvG1 | Acetolactate synthase II, large subunit, interrupted | 0.513 | |
| trpE | Anthranilate synthase component I | 0.511 | |
| metE | Tetrahydropteroyltriglutamate methyltransferase | 0.499 | |
| cysK | Cysteine synthase A, O-acetylserine sulfhydrolase A | 0.497 | |
| aspC | Aspartate aminotransferase | 0.482 | |
| trpA | Tryptophan synthase, alpha protein | 0.481 | |
| argA | N-Acetylglutamate synthase | 0.444 | |
| pheA | Chorismate mutase P and prephenate dehydratase | 0.439 | |
| serC | 3-Phosphoserine aminotransferase | 0.401 | |
| Vitamins and cofactors | nrdH | Glutaredoxin-like protein; hydrogen donor | 0.638 |
| hemC | Porphobilinogen deaminase | 0.552 | |
| entE | 2,3-Dihydroxybenzoate-AMP ligase | 0.543 | |
| grxB | Glutaredoxin 2 | 0.506 | |
| gst | Glutathionine S-transferase | 0.501 | |
| folE | GTP cyclohydrolase I | 0.489 | |
| ggt | Gamma-glutamyltranspeptidase | 0.477 | |
| entF | ATP-dependent serine activating enzyme | 0.459 | |
| entB | 2,3-Dihydro-2,3-dihydroxybenzoate synthetase, isochorismatase | 0.451 | |
| entC | Isochorismate hydroxymutase 2 enterochelin biosynthesis | 0.428 | |
| thiH | Thiamine biosynthesis | −0.473 | |
| Nucleotides | pyrI | Aspartate carbamoyltransferase, regulatory subunit | 0.628 |
| pyrB | Aspartate carbamoyltransferase, catalytic subunit | 0.464 | |
| prsA | Phosphoribosylpyrophosphate synthetase | −0.472 | |
| ndk | Nucleoside diphosphate kinase | −0.484 | |
| pfs | ORF, hypothetical protein | −0.515 | |
| gmk | Guanylate kinase | −0.646 | |
| upp | Uracil phosphoribosyltransferase | −0.646 | |
| Fatty acid and phospholipid metabolism | cfa | Cyclopropane fatty acyl phospholipid synthase | 0.618 |
| fadA | 3-ketoacyl-CoA thiolase | 0.494 | |
| fabA | β-Hydroxydecanoyl thioester dehydrase | −0.413 | |
| fabD | Malonyl-CoA-[acyl-carrier-protein] transacylase | −0.437 | |
| fabI | Enoyl-[acyl carrier protein] reductase | −0.468 | |
| accC | Acetyl-CoA carboxylase, biotin carboxylase subunit | −0.557 | |
| fabH | 3-Oxoacyl-[acyl carrier protein] synthase III | −0.571 | |
| fabF | 3-Oxoacyl-[acyl carrier protein] synthase II | −0.576 | |
| fabZ | (3R)-Hydroxymyristol acyl carrier protein dehydratase | −0.608 |