Figure 16.

(A) Representative images showing the enzymatic activity of L-alanine aminopeptidase, L-leucine aminopeptidase, and alkaline phosphatase in RTpiMI cells cultured on ThinCert^® (TC) inserts, on TC coated with Matrigel^® (MM), on MM embedded with fibroblasts (MMfb), on Alvetex™ scaffold (AV), and in the RT intestine. Quantification of the enzymatic activity of alkaline phosphatase (B) and alanine aminopeptidase (C) in RTpiMI epithelial cells cultured in combination with increasingly complex in vitro platforms. Values are expressed as means ± SD. Differences were considered statistically significant if p < 0.05 (different superscripts within the same graph indicate statistically significant differences).