TABLE 1.
No. | Gene or ORF designation | Function [hypothetical]b | MW | pI | ORF | Reference |
---|---|---|---|---|---|---|
1 | FAA1 | Long-chain fatty acid CoA ligase (for degradation or incorporation in PL) | 77,866 | 7.70 | YOR317c | 23 |
2 | FAT1 | Very long-chain fatty acid activator; fatty acid transport protein (exogenous long-chain fatty acids) | 71,700 | 8.40 | YBR041c | 10, 40 |
3 | FAA4 | Long-chain fatty acid CoA ligase (for degradation or incorporation into PL) | 77,267 | 6.33 | YMR246w | 22 |
4 | ERG7 | Lanosterol synthase | 83,318 | 6.30 | YHR072w | 4 |
5 | YMR313c | Unknown | 73,612 | 8.74 | YMR313c | |
6 | TGL1 | [Triacylglycerol lipase] | 62,979 | 6.60 | YKL140w | 1 |
7 | ERG1 | Squalene epoxidase | 55,120 | 6.27 | YGR175c | 19, 28 |
8 | YBR177c | [UFP007 family (not characterized); weak homologue to probable membrane receptor] | 51,255 | 7.84 | YBR177c | |
9 | YOR059c | [Lipase serine active site] | 51,114 | 9.20 | YOR059c | |
10 | YIM1 | [Homologue to mitochondrial inner membrane protease] | 41,651 | 8.24 | YMR152w | |
11 | ERG6 | Sterol-Δ24-methyltransferase | 43,421 | 5.59 | YML008c | 27 |
12 | YDL193w | [Weak homologue to Ca2+ channel protein] | 42,555 | 6.73 | YDL193w | |
13 | TDH1 | GAPDH | 35,619 | 8.41 | YJL052w | 32 |
TDH2 | 35,715 | YJR009c | ||||
TDH3 | 35,615 | YGR192c | ||||
14 | YJU3 | [Lysophospholipase] | 35,549 | 8.55 | YKL094w | 12 |
15 | YIM4 | [Oxidoreductase (short-chain dehydrogenase or reductase family)] | 32,822 | 9.34 | YIL124w | |
16 | YKR0456 | Unknown | 31,246 | 8.85 | YKR046c | |
SLC1c | 1-acylglycerol-3-phosphate acyltransferase | 33,903 | 9.76 | YDL052c | 3, 34 |
MW, molecular weight; pI, isoelectric point; PL, phospholipid.
Functions in brackets are hypothetical.
The gene product of SLC1 was not detected during the mass spectrometric approach described in this work but was characterized earlier by biochemical and molecular biological means.