TABLE 2.
Characterization of gene products present in yeast lipid particles
| No. | Gene or ORF designation | Gene product
|
Deletion strain phenotype | |
|---|---|---|---|---|
| TM | Hydrophobicity domain ≥2a | |||
| 1 | FAA1 | 1 | 2 (middle and C terminal) | Viable |
| 2 | FAT1 | 3 | 2 (N terminal and middle) | Viable |
| 3 | FAA4 | 0 | 2 (middle and C terminal) | Viable |
| 4 | ERG7 | 0 | 1 (C terminal) | Sterol auxotroph |
| 5 | YMR313c | 0 | 1 (C terminal) | Viable |
| 6 | TGL1 | 1 | 1 (N terminal) | Viable |
| 7 | ERG1 | 2 | 3 (N and C terminal) | Sterol auxotroph |
| 8 | YBR177c | 0 | 1 (middle) | Viable (temperature sensitive) |
| 9 | YOR059c | 1 | 1 (middle) | Viable |
| 10 | YIM1 | 0 | 0 | Viable |
| 11 | ERG6 | 0 | 0 | Viable |
| 12 | YDL193w | 1 | 1 (middle) | Lethal |
| 13 | TDH1 | 0 | 0 | Viable |
| TDH2 | 0 | 0 | Viable | |
| TDH3 | 0 | 0 | Viable | |
| 14 | YJU3 | 0 | 0 | Viable |
| 15 | YIM4 | 0 | 2 (middle and C terminal) | Viable |
| 16 | YKR0456 | 1 | 0–1 | Viable |
| SLC1 | 1 | 2 (N terminal) | Viable | |
a
Hydrophobicity index from Kyte and Doolittle plots (25); the positions of the hydrophobic domains within the polypeptide are shown in parentheses.