Figure 3.
Protein O–GlcNAcylation mediated by Aβ1–42 oligomers control microglia migration: Quantitative and qualitative analysis of microglia migration assessed by an in vitro transwell assay. Microglia were treated with either a vehicle (DMSO), OSMI–I or Thiamet G and were either stimulated or not with 5 µM Aβ1–42 oligomers. Cell migration was analyzed after 24 h of incubation. (A–F) Representative photographs of a randomly chosen field of one independent experiment out of three. Purple areas correspond to all the cells that migrated, as stained with Mayer’s hematoxylin. (G) The bar graph represents the quantification of the migration. Values indicate the mean ± S.E.M. of the stained area (corresponding to the migrated cells) expressed as the % of control in four randomly chosen fields of three independent experiments (N = 3). Mann–Whitney statistical analysis showed a significant difference. *** p < 0.001.