TABLE 1.
Summary of results from N-terminal sequencing and MALDI
| Spot no. | N-terminal sequence | Peptide massesa | Corresponding peptide fragment sequencesa | MCSP contained in spot |
|---|---|---|---|---|
| 1 | SNKMTGLVKWFDAGKG | NDb | B, A1 | |
| 2 | SNKMTGLVKWFNADKG | 723.3 | 11-KWFDAGKG-16 (CspB or CspA1) | A1, A2, B |
| MSNKMTGLVKWFNADKG | 780.3 | 11-KWFNADKG-16 (CspA2) | ||
| 926.5 | 61-KGPSAVNVVAL-70 (CspA1) | |||
| 940.5 | 61-KGPSAVNVIAL-70 (CspA2) | |||
| 3 | SNKMTGLVKWFNADKG | 780.3 | 11-KWFNADKG-16 (CspA2) | A2 |
| 940.5 | 61-KGPSAVNVIAL-70 (CspA2) |
a
Peptide masses and fragments from MALDI are examples showing fragments at the relevant amino acid signatures which are specific for CspA1, CspA2, and CspB (6), respectively.
b
ND, not determined.