Figure 2. STAT3 deficiency in donor T cells preserves GVL activity.

Lethally irradiated WT BALB/c recipients were engrafted with TCD-BM cells (5 × 10⁶) from WT C57BL/6 donors with or without CD90.2⁺ T cells (2.5 × 10⁶ [A–F] or 1 × 10⁶ [C and D]) from STAT3^–/– C57BL/6 donors on day 0. Recipients were then challenged with i.p. injection of BCL1/Luc cells (625 × 10³ [A and B] per mouse) or i.v. injection of ALL/Luc cells (40 × 10³ [C and D] or 10 × 10³ [E and F] per mouse) on day 0. (A, C, and E) Mice were monitored for tumor growth by using in vivo BLI after HCT. Representative BLI images of each mouse from each time point are shown. (B, D, and F) Curves of photon/second and percentage of tumor-free survival are shown. Data are represented as mean ± SEM. n = 9–10 per group combined from 2 replicated experiments. Crosses indicate deaths. P values were calculated using nonlinear regression (curve fit) for photon/second comparisons (B, D, and F) and log-rank test for survival comparisons (B, D, and F). **P < 0.01, ****P < 0.0001.