Figure 3. Innate type 2 immune responses are exaggerated in Plscr1-null mice.

(A) Gating strategy for ILC2 cells in mouse lung. ILC2 are defined as Lin-Thy1.2⁺ICOS⁺T1/ST2⁺ cells. (B) WT and Plscr1^–/– mice were subjected to HDM administration (25 μg HDM 3 times a week for 3 weeks), and lung ILC2 recovery was assessed by flow cytometry. (C and D) Whole lung mRNA was extracted and IL-25 mRNA (C) and IL-33 mRNA (D) were assessed by RT-PCR. (E) BAL SLPI levels were measured by ELISA. (F and G) ILC2 cells were isolated as described in methods. mRNA was extracted from primary ILC2s; IL-25R mRNA (F) and IL-33R mRNA (G) were assessed by RT-PCR. (H) BAL soluble IL-33R/ST2 levels were measured by ELISA. Values are mean± SEM with 7–9 mice in each group. Comparisons between groups were conducted by 2-way ANOVA with Bonferroni’s posthoc test. *P ≤ 0.05, **P ≤ 0.01.