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. 2023 Aug 1;133(15):e169583. doi: 10.1172/JCI169583

Figure 6. The effects of Plscr1 on ILC2 and IT2IR are mediated via CRTH2.

Figure 6

WT,CRTH2–/–, Plscr1–/–, and CRTH2–/–Plscr1–/– mice were subjected to HDM administration. (A) BAL eosinophil counts were assessed by Diff-Quik staining. (B) Lung ILC2 recovery was assessed by flow cytometry. (C and D) ILC2s were sorted and mRNA was extracted from ILC2s, and IL-25R (C) and IL-33R (D) mRNA were assessed by RT-PCR. (E and F) ILC2 were sorted and mRNA was extracted from ILC2s, and IL-13 (E) and IL-5 (F) mRNA were assessed by RT-PCR. (G and H) ILC2 were sorted and cultured for 2 days, and IL-13 (G) and IL-5 (H) protein levels were assessed by ELISA. Values are mean± SEM with a minimum of 4 mice in HDM group. Comparisons between groups were conducted by 2-way ANOVA with Bonferroni’s posthoc test. *P ≤ 0.05,**P ≤ 0.01.