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. 2023 Jul 20;12(14):1899. doi: 10.3390/cells12141899

Figure 3.

Figure 3

Impact of TIMP-1 and a non-MMP inhibitory mutant (Δvv-TIMP-1) on the internalization of cell surface CD74 and CXCR4 in THP-1 cells. CD74 and CXCR4 cell surface expression were monitored using FITC-conjugated anti-CD74 and APC-labeled anti-CXCR4 antibodies, respectively. Full-length TIMP-1 (wtTIMP-1; (a,b)) promotes the internalization of CD74 in THP-1 cells in a dose-dependent manner (minimum at 100 ng/mL). Δvv-TIMP-1 (c,d) also enhances the internalization of CD74, but the effect is smaller and lacks a dose behavior. (a,c) Representative histograms. (b,d) Quantification of CD74 cell surface expression from three independent experiments. MIF (200 ng/mL) was used as a positive control. (e,f) Stimulation of THP-1 with TIMP-1 showed no effect on CXCR4 internalization. (e) Representative histogram, (f) Quantification of CXCR4 cell surface expression from three independent experiments. Data are means ± SD.