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. 2023 Jul 22;15(14):3721. doi: 10.3390/cancers15143721

Figure 4.

Figure 4

Hu2EF engineered variants binding properties. Flow cytometry analysis of Trop-2-expressing cancer cells, stained with the murine 2EF-Alexa488 mAb, as competed-out by unlabeled murine 2EF (Competitor: murine), unlabeled Hu2EF-6 (Competitor: Hu2EF-6) or unlabeled Hu2EF-7 (Competitor: Hu2EF-7). Competing mAb were added at the indicated ratios (color-coded from 1× to 27×) to fixed amounts of 2EF-Alexa488. Stained cancer cells were, from top to bottom, KM12SM/Trop-2, Colo205, HT29, DU-145 and MCF-7. Relative affinity correlates with the reduction in fluorescence signals. Unstained controls are in black.