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Accumulation and modification of PII and glutamine synthetase in R. rubrum. Extracts from cells grown under N+ or N− conditions were subjected to SDS-PAGE and Western blotting. B1 denotes strain S1MJ, carrying pSNCB1, and B1A denotes strain S1MJ, carrying pSNCB1A. The left panel shows glutamine synthetase and the right panel shows PII. Upper, adenylylated form of glutamine synthetase, lower, unmodified form of glutamine synthetase.
