TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− Δ Φ80d lacZΔM15 endA1 recA1 hsdR17(rm− mK+) supE44 thi-1 gyrA96 Δ(lacZYA-argF)U169 | 7 |
| S17-1 | Smr Spr Tra+; pro thi hsdR recA; chromosomal integration of RP4-2-Tc::Mu-Kan::Tn7 | 27 |
| R. rubrum | ||
| S1MJ | Smr; a spontaneous strain of S1 | This laboratory |
| UR381 | Kmr Smr; Δ ntrBC1::Kan | 30 |
| Plasmids | ||
| pRKD418 | Tcr Tpr; cloning vector | 17 |
| pGEM-luc | Apr; source for luc | Promega |
| pJJ303 | Apr; pGEM-luc derivative containing tac promoter upstream of luc | 22 |
| pJOM | Apr; pGEM3zf(+) derivative containing glnB, glnA, and flanking regions | 9 |
| pJCN100 | Apr; pJJ303 derivative containing glnB and glnA | This work |
| pSNC208 | Tcr Tpr;pRKD418 digested by BamHI-XhoI and ligated with BamHI-XhoI luc from pGEM-luc | This work |
| pRKD419 | Tcr Tpr; pRKD418 with a complete BgIII-BamHI digestion and religation | This work |
| pSNC109 | Tcr Tpr; pRKD419 digested by NsiI-XhoI and ligated with NsiI-XhoI luc from pGEM-luc | This work |
| pSNCBP1 | Tcr Tpr; pSNC109 derivative containing glnBp1 | This work |
| pSNCB1 | Tcr Tpr; pSNC109 derivative containing glnBp1 and glnBp2 | This work |
| pSNCB2 | Tcr Tpr; pSNC109 derivative containing glnBp2 | This work |
| pSNCB1A | Tcr Tpr; pSNC109 derivative containing glnBp1 and glnBp2, glnB and upstream of glnA | This work |
| pSNCB2A | Tcr Tpr; pSNC109 derivative containing glnBp2, glnB and upstream of glnA, | This work |
| pSNCA1+ | Tcr Tpr; pSNC208 derivative containing glnB and upstream of glnA | This work |
| pSNCA1− | Tcr Tpr; same as pSNCA1+ but with insertion in opposite direction | This work |
| pSNCglnA | Tcr Tpr; pSNC109 derivative containing 189-bp 3′-end of glnB and upstream of glnA | This work |
| pSNCref | Tcr Tpr; pSNC109 derivative containing 53 bp upstream of glnA | This work |