Figure 5.
Deficiency of endothelial MLKL downregulates the liver fibrosis indexes in CCl4 model. (A) RT-qPCR of Col1a1, Col3a1, and α-SMA expression in liver tissues of mock and fibrosis mice. n ≥ 3 per group. (B) Liver hydroxyproline amounts were detected in control versus Mlkl+/+ and MlkliΔEC/iΔEC mice of NASH model. n ≥ 3 per group. (C) α-SMA and Collagen I protein abundance in liver tissues of indicated mouse groups in the NASH model as indicated by Western blot and quantification (D). n ≥ 3 per group. (E,F) Immunostaining for Collagen I (red), Desmin (green), and DAPI (blue) in Mlkl+/+ and MlkliΔEC/iΔEC mice that were subjected to CCl4 injections. Left: ROI (region of interest), scale bars, 200 μm. Right: Magnified images of indicated dotted box areas, scale bars: 25 μm. (G) Mean staining was quantified by image J. n ≥ 3 per group. Data are shown as means ± SEMs. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.