TABLE 1.

Localization of proteins in A. faecalis

Cell fractiona	% Total recovery (n = 4)
	AADHb	Azurinc	Cytochromed	Malate dehydrogenasee
Periplasm	73.9 ± 2.5	76.5 ± 3.1	100	7.5 ± 0.9
Cytoplasm	24.3 ± 2.1	24.5 ± 0.3	0	89.8 ± 3.1
Membrane	1.6 ± 0.2	NDf	0	2.6 ± 0.1

^aCells were fractionated as described in Fig. 1. 

^bThe steady-state assay for AADH activity was performed as described previously (12). 

^cThe amount of purified azurin was quantitated using the extinction coefficient ɛ₆₀₀ = 4,000 M⁻¹ cm⁻¹ (13). 

^dCytochrome c levels were quantitated by performing densitometry of the heme-stained proteins after SDS-PAGE. 

^eThe assay of malate dehydrogenase was performed as described previously (1). 

^fND, it was not possible to determine what azurin if any remains associated with the membrane fraction as it is a soluble protein that can be detected only spectrophotometrically.