Figure 8.

NO plus TMZ cotreatment decreases active caspase 3. Representative Western blot showing the expression of active caspase 3 in GSCs after 21 days of treatment with 50 µM TMZ, or DMSO at the same dilution, in the presence (right) or absence (left) of NO. TMZ and DMSO administration was started 7 days after NO treatment. The level of active caspase 3 was normalized to β-actin and the mean ± SEM of the densitometric analysis of 2 experiments is recorded. Left bar shows the effect of TMZ alone calculated with respect to DMSO. The middle bar shows changes induced by TMZ plus NO cotreatment with respect to NO single administration. The right bar shows the effect of combined treatment with respect to TMZ administration alone. * p < 0.05 vs. NO-treated cells; # p < 0.05 vs. TMZ-treated cells.