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. Author manuscript; available in PMC: 2023 Jul 28.
Published in final edited form as: ACS Biomater Sci Eng. 2022 Oct 3;8(11):4942–4955. doi: 10.1021/acsbiomaterials.2c00851

Figure 4.

Figure 4.

Intestinal epithelial cells (Caco2 and HT29-MTX) formed a confluent and functional monolayer in 3D silk half-scaffolds. Schematic of Caco2/HT29-MTX monolayer and primary myofibroblasts. (A–F) Representative confocal z-stack of the immunostained luminal surface of the scaffold (scale bar: 200 μm). Confocal immunofluorescence images of the epithelia for ZO-1 (A, B), Muc-2 (C, D), and SI (E, F). Scale bars: 20 μm. (A, C, E) 3D reconstruction of z-stack of fluorescence microscope images of the scaffolds. (B, D, F) Confocal maximum projection fluorescence microscope images of the scaffolds with higher magnification on the luminal surface.