Figure 3.

Heat map and correlation analysis of antioxidative−related parameters (A,B) and Nrf2 protein levels (C,D) in the skin of on−growing grass carp (Ctenopharyngodon idella) administered vitamin D after infection with A. hydrophila. Nrf2, nuclear factor E2−related factor 2; Keap1, kelch−like ECH−associated protein 1. N = 6 for gene expression and N = 3 for Western blotting analysis. Different letters above bars indicate significant differences (p < 0.05); red indicates upregulation; blue indicates downregulation; * presents p < 0.05, ** presents p < 0.01.