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Gene expression analysis by qPCR of CCND1, CCNE, and CCNA1 and protein levels by Western blot of cyclin D, cyclin E, and cyclin A1 in LXF-289 (A,B) and SK-MES-1 cell lines (C,D). The statistical analysis of protein expression changes was based on densitometry results obtained from three independent experiments. The asterisk indicates p < 0.05.
