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. 2023 Jul 19;12(14):2740. doi: 10.3390/foods12142740

Figure 7.

Figure 7

COS ameliorated LPS-activated Erk1/2 and Akt induction in RAW264.7 cells. The cells were pretreated with 500 μg/mL COS for 24 h, followed by with or without the presence of signaling molecule inhibitor (10 μM PD98095 Erk1/2 inhibitor, 5 μM Perifosine Akt inhibitor) and 1 μg/mL LPS 24 h. Protein expressions were performed by western blot analysis. (a) Expression of Erk1/2 and p-Erk1/2. (b) Quantification of p-Erk1/2/Erk1/2. (c) Expression of Akt. (d) Quantification of pAkt/Akt. Quantification of the proteins is reported as the relative of the proteins to beta actin. Results are shown as the mean ± SD (n = 4). * p < 0.05 vs. control cells. # p < 0.05 vs. the LPS group.