FIG. 2.

Northern blot analysis of RNA isolated from L. fermentum BR11 (A) and L. rhamnosus GG (B) harboring the slpA wild-type and derivative promoter constructs. Membranes were hybridized with DIG-labelled gusA and 16S rRNA probes as described in Materials and Methods. Lanes 1, pNZslp (wild type); lanes 2, pNZTG; lanes 3, pNZT10; lanes 4, pNZT35; lanes 5, pNZT1035; lanes 6, pNZS10; lanes 7, pNZS35; lanes 8, pNZS1035. Signals were quantified by densitometry and normalized for relative plasmid copy number. The relative transcriptional activities determined are given in Table 1.