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TABLE 1.

slpA promoter base substitution mutants and relative transcriptional activities in Lactobacillus strains

Plasmid Sequence Relative gusA transcript levela
L. fermentum BR11 L. rhamnosus GG
−35 TG  −10
pNZslpb TTGCTATTTCTTGAAGAGGTTAGTACAAT 100 100 (450)
pNZS10 -------------------------T--- 412* 7.5** (34)
pNZS35 ---AC------------------------ 426** 33** (149)
pNZS1035 ---AC--------------------T--- 312 100 (450)
pNZTG ---------------------G------- 1,072** 15** (68)
pNZT10 ---------------------G---T--- 500** 16** (72)
pNZT35 ---AC----------------G------- 116 16** (72)
pNZT1035 ---AC----------------G---T--- 131 212**(954)
a

Represented as percent wild-type activity (values in parentheses represent L. rhamnosus GG transcript levels normalized to those of L. fermentum BR11). Significance levels (Student’s t test) are as follows: *, P < 0.05; **, P < 0.01. Values in boldface type are maximal gusA mRNA levels in each strain. 

b

Wild type.