TABLE 1.
Plasmid | Sequence | Relative gusA transcript levela
|
|
---|---|---|---|
L. fermentum BR11 | L. rhamnosus GG | ||
−35 TG −10 | |||
pNZslpb | TTGCTATTTCTTGAAGAGGTTAGTACAAT | 100 | 100 (450) |
pNZS10 | -------------------------T--- | 412* | 7.5** (34) |
pNZS35 | ---AC------------------------ | 426** | 33** (149) |
pNZS1035 | ---AC--------------------T--- | 312 | 100 (450) |
pNZTG | ---------------------G------- | 1,072** | 15** (68) |
pNZT10 | ---------------------G---T--- | 500** | 16** (72) |
pNZT35 | ---AC----------------G------- | 116 | 16** (72) |
pNZT1035 | ---AC----------------G---T--- | 131 | 212** (954) |
Represented as percent wild-type activity (values in parentheses represent L. rhamnosus GG transcript levels normalized to those of L. fermentum BR11). Significance levels (Student’s t test) are as follows: *, P < 0.05; **, P < 0.01. Values in boldface type are maximal gusA mRNA levels in each strain.
Wild type.