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. 2023 Jul 19;24(14):11652. doi: 10.3390/ijms241411652

Figure 4.

Figure 4

Purification of bacteriolytic enzymes Serp and Serp3 from the culture fluid of L. capsici expression strains. (a) Serp and Serp3 purification schemes. Red arrows indicate the Serp purification scheme; green arrows indicate the Serp3 purification scheme. (b) SDS-PAGE: 1, 4, 6 and 8, markers; 2, the culture fluid of wild-type L. capsici (Supplementary File S1 Figure S5); 3, the culture fluid of L. capsici PGro(A)–serp3(6his) (Supplementary File S1 Figure S5); 5, the culture fluid of L. capsici PT5–serp(6his) (Supplementary File S1 Figure S6); 7, purified Serp3 of L. capsici PGro(A)–serp3(6his) (0.18 μg) (Supplementary File S1 Figure S7); 9, purified Serp of L. capsici PT5–serp (0.35 μg) (Supplementary File S1 Figure S8). A 12 μL amount of the preparations was applied to the electrophoresis.