Table 7.
Oligonucleotides used in this work.
| Oligonucleotides | Sequence | Goal |
|---|---|---|
| Oligonucleotides used for cloning | ||
| Serp_BamHI (for) | GGATCCATGATCCGCAAGAACGCACTTTG | To amplify gene serp(6his) 1395 bp long with DNA of L. capsici VKM B-2533T |
| Serp_HindIII (rev) | AAGCTTTCAATGATGATGATGATGATGGGGATTGAAATAGCTCGACACG | |
| Serp3_HindIII (for) | AAGCTTATGCGTAAGTTCAGTTTGTCGATCCTC | To amplify gene serp3(6his) 1233 bp long with DNA of L. capsici VKM B-2533T |
| Serp3_BamHI (rev) | GGATCCTTAATGATGATGATGATGATGCGGACTGGTGCG | |
| Oligonucleotides used for sequencing | ||
| T5_KpnI (for) | GGTACCGTGCCACCTGACGTCTAAG | To confirm absence of mutation in the cloned sequence in the assembled pBBR1-MCS5 PT5–serp(6his) constructs |
| T5_XbaI (rev) | TCTAGACTGAAAATCTCGCCAAGCTAGC | |
| Gro_KpnI (for) | GGTACCCGGACCGACGCCTGTCA | To confirm absence of mutation in the cloned sequence in the assembled pBBR1-MCS5 pBBR1-MCS5 PGroEL(A)–serp3(6his) constructs |
| Term_XbaI (rev) | TCTAGAAGAGTTTGTAGAAACGCAAAAAGGC | |