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. 2023 Jul 23;24(14):11823. doi: 10.3390/ijms241411823

Figure 1.

Figure 1

The morphology, mRNA, and protein expressions of FE25, FE25-lenti-ctrl, FE25-PRA, and FE25-PRB cells. (A) The morphology of various cells. The cell morphology was not changed after PRA or PRB transfection. The cell morphology revealed a cuboidal shape, which was characteristic of epithelial cells. Scale bar = 100 μm. (B) qPCR showed PRA and PRB expressions after transfection (n = 3). *** p < 0.001 when compared to FE25. FE25-PRA increased PRA expression. FE25-PRB increased both PRA and PRB expressions. (C) Western blot of PRA and PRB after PRA and PRB transfections. FE25-PRA increased PRA expression. The Western blot of PRB in FE25-PRB was performed at different passages (P3 and P14), which showed constant expression of PRB at different passages. PRA, progesterone receptor A; PRB progesterone receptor B; and qPCR, quantitative polymerase chain reaction.