FIG. 2.
Immunoblot analysis of the single-substitution mutants. A single set of blots was exposed to antiserum against p6Gag (A) and then successively stripped and reprobed with antiserum to p24CA (B). The virion samples produced by transfection (corresponding to ∼1.2 × 107 cpm of reverse transcriptase activity) are identified above each lane. sssDNA, mock control; WT, sample of the wild-type NL4-3 virions. The apparent molecular masses as determined by relative mobility are presented at the left.