FIG. 4.

Immunoblot analysis of Y36S-L41P and Y36F virions. (A) Blots of both wild-type NL4-3 (WT) and Y36S-L41P virion samples produced from transfection (corresponding to ∼1.2 × 10⁷ cpm of reverse transcriptase activity) as well as samples of lysates isolated from cells transfected with viral constructs (2% of the lysate from a confluent T150 flask). (B) Immunoblots of wild-type and Y36F virions samples produced from transfection (corresponding to ∼1.2 × 10⁷ cpm of reverse transcriptase activity). Samples are identified above the lanes, and the antiserum used for each reaction is noted above each blot. Molecular masses as determined by relative mobility are presented on the left.