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. 2023 Jul 22;24(14):11781. doi: 10.3390/ijms241411781

Figure 1.

Figure 1

Figure 1

The effect of SAS on the intracellular GSH levels and ROS accumulation in OCCC cell lines. (A) GSH levels were compared with or without 400 μM SAS treatment for 24 h in OCCC cells. GSH levels were calculated from the ratio of cells treated with drugs to that of untreated cells set as 1 (mean ± SD; n = 8). Data are presented as the mean ± standard deviation (SD). * p < 0.05 vs. un-treated cells. (B) OCCC cells were treated with 400 μM SAS for 24 h and the intracellular ROS levels were analyzed by means of flow cytometry after staining with DCFH-DA. GSH, glutathione; ROS, reactive oxygen species; SAS, sulfasalazine; DCFH-DA, 2′,7′-dichlorofluorescin diacetate. −, without; +, with.