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Immunofluorescence detection of A2B5 (red) expression on U87-ST8Sia3 cells before and after neuraminidase administration at 6, 24 and 72 h. Hoechst staining of the cell nuclei (blue) is also shown. Scale bar = 10 μm (a). Quantification of A2B5 expressing by flow cytometry in GBM cell lines treated with neuraminidase or vehicle (control) for 24 h (b).
