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. 2023 Jul 19;24(14):11645. doi: 10.3390/ijms241411645

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Hydrophilic interaction liquid chromatography–triple quadrupole mass spectrometry total ion chromatograms (TIC) of the standard mixture (GM3, GM2, GM1, GD3, GD2, GD1a, GD1b, GT1b and GQ1b) (a), U87-MG (b), U87-ST8Sia3 with or without neuraminidase (Neura) administration (c,d), GBM6 CSC line with or without neuraminidase administration (e,f) and GBM9 CSC line with or without neuraminidase administration (g,h). The mass spectrometer was operated in the negative precursor ion mode of the ion m/z 290 corresponding to N-acetyl neuraminic acid.