Figure 4.

Platelet counts, D-dimer, and ADAMTS-13 activity in endotoxemic mice: (A) platelet counts were measured on an ABX Micros 60 Human Blood analyzer 30 min, 24 h, 72 h, and 7 days after LPS injection (n = 10/group, student’s t-test, * p < 0.05). (B) Plasma levels of D-dimer measured at 72 h post-LPS treatment (n = 10/group, one-way ANOVA). (C) ADAMTS-13 activity was measured by a flow-based assay. Plasma from surviving LPS-treated mice at the end of 7 days was mixed with human platelets and perfused over histamine-activated HUVECs for 2 min at 2.5 dynes/cm². The number of ULVWF strings remaining at the end of perfusion was counted (n = 10/group, one-way ANOVA).