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. 2023 Jul 13;24(14):11421. doi: 10.3390/ijms241411421

Figure 5.

Figure 5

Systemic delivery of dual AAV qDys in mdx mice improves muscle pathology and muscle function. (A) Representative sirius red and hematoxilin and eosin staining performed on GA and DIA sections of DBA2 wt, DBA2 mdx and DBA2 mdx that had been systemically treated with dual vector for 8 weeks. Arrows indicate inflammatory and necrotic area. (B) Sirius red analysis was performed using QuPath-0.3.2 software and Centro-nucleation analysis was performed with ImageJ open-source software and R studio. Centronucleation is expressed as the percentage of myofibers with centrally located nuclei relative to total number of fibers. (C) Creatine kinase levels (U/l) calculated from serum collected 8 weeks after AAV injection. (D) Grip test (performed 2 days before euthanasia) and Escape test (day of sacrifice) performed in wt, mdx and mdx where the dual vector was delivered (mdx + qDys). Force values were normalized for body weight (N/g). Data are presented as mean ± SEM (n = 6) (** p ≤ 0.01, **** p ≤ 0.001).