Skip to main content
. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Cancer Lett. 2023 Jul 6;568:216304. doi: 10.1016/j.canlet.2023.216304

Figure 1. One-carbon metabolism genes are overexpressed in anaplastic thyroid cancer.

Figure 1.

A) Hierarchical clustering analysis of gene expression for one-carbon, purine synthesis, and thymidine synthesis genes from wild type thyroids and ATCs developed by [Pten,Tp53]−/− mice. B) Western blot analysis of protein extracts from wild type thyroids and ATCs developed by mice of the indicated genotypes. C) Violin plot analysis of scRNAseq data from normal human thyroids and samples from PTC and ATC patients. D) Western blot analysis of the expression of SHMT1, SHMT2, and MTHFD2 in a panel of human ATC cells lines. Immortalized NTHY-ori cells are included as a comparison for non-transformed cells. E) Graphic representation of the expression levels of serine synthesis, one-carbon, purine synthesis, and thymidine synthesis genes as detected by microarray expression analysis. In yellow, genes overexpressed only in mouse ATCs, in blue genes overexpressed only in human ATCs, and in red genes overexpressed in both mouse and human ATCs. F) Analysis of TCGA data from PTC patients, showing that overexpression of SHMT2 and/or MTHFD2 correlates with reduced progression-free survival.