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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Cancer Lett. 2023 Jul 6;568:216304. doi: 10.1016/j.canlet.2023.216304

Figure 5. Functional analysis of one-carbon pathway inhibition in BHT101 cells.

Figure 5.

A) Rescuing effect of exogenous formate addition on the growth inhibition induced by one-carbon pathway inhibitors. B) Exogenous hypoxanthine, but not thymidine, rescues the growth inhibition induced by one-carbon pathway inhibitors. C) Lethal effect of exogenous glycine deprivation combined with SHIN1, and rescuing effect of exogenous glycine added to the medium. D) Systematic analysis of the extent of viability rescue by exogenous formate, hypoxanthine, and glutathione (GSH) in the presence and absence of extracellular glycine. Dotted lines correspond to viability levels with and without glycine upon SHIN1 treatment. E) Analysis of the ability of exogenous formate, hypoxanthine, and thymidine to rescue the inhibitory effect of SHIN1 in cells depleted of SHMT1 or SHMT2. F) Growth suppressive effect of combining one-carbon pathway inhibitors with different concentrations of methotrexate (MTX) and pemetrexed (PEM). In all graphs, significant (P<0.05) differences relative to the corresponding DMSO controls are indicated by an asterisk.