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. 2023 Jul 22;24(14):11783. doi: 10.3390/ijms241411783

Figure 4.

Figure 4

Homology analysis of FWL proteins in different species. Multiple sequence alignments of FWL proteins were performed using the MUSCLE alignment function in MEGA (Version 11) with default settings. The maximum-likelihood method of the IQ-TREE function in TBtools (v1.09876) software was applied to construct the phylogenetic tree with 1000 bootstrap replicates. The FWL proteins were used to run 1000 self-replicates and build a phylogenetic tree using the neighbor-joining method on the MEGA (Version 11) program. Based on branching, the FWL family proteins were classified into five subfamilies. Each subfamily is represented by a distinct color: purple for subfamily A, red for subfamily B, yellow for subfamily C, blue for subfamily D, and green for subfamily E. Sl (Solanum lycopersicum) stands for tomato, At (Arabidopsis thaliana) for Arabidopsis, Ca (Capsicum annuum) for pepper, Gm (Glycine max) for soybean, Os (Oryza sativa) for rice and Zm (Zea mays) for maize.