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. 2023 Jul 24;24(14):11860. doi: 10.3390/ijms241411860

Figure 7.

Figure 7

Antibody-mediated blocking of clathrin has no effect in blocking S protein pseudovirion infection of ARPE-19 cells. (A) Clathrin is expressed in ARPE-19 cells (*; red). Immunoblot of clathrin with anti-clathrin HC antibody (red) and β-actin (green) in ARPE-19 cells. (B) Fold difference in uptake of original spike-pseudotyped lentiviruses at MOI 10 in the presence of anti-clathrin antibody at 1:500 dilution (1 µg/mL) and 1:250 dilution (2 µg/mL) at 24 h compared to untreated controls infected with original spike protein pseudotyped particles alone. **** p < 0.0001, ns—p > 0.05.