Figure 10.

Antibody-mediated blocking of flotillin-1 has no effect in blocking S protein pseudovirion infection of ARPE-19 cells. (A) Flotillin-1 is expressed in ARPE-19 cells; immunoblot visualized with anti-flotillin-1 antibody (*; green). (B) Fold difference in uptake of original spike-pseudotyped lentiviruses at MOI 10 in the presence of anti-flotillin antibody at 1:500 dilution (1 µg/mL) and 1:250 dilution (2 µg/mL) at 24 h compared to untreated controls infected with original spike protein pseudotyped particles alone. **** p < 0.0001, ns—p > 0.05.