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. 2023 Jul 19;24(14):11635. doi: 10.3390/ijms241411635

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of IFN-γ-primed iPSC-derived MSCs (iMSCs) and verification of indoleamine 2,3-dioxygenase (IDO) expression. (A) Light microscopic image analysis of the morphology of IFN-γ-primed iMSCs. (B) Flow cytometry analysis of IFN-γ-primed iMSCs for surface markers related to exosomes (CD73, CD90, and CD105) and those not related to exosomes (CD34 and CD45). (C–E) mRNA and protein levels of IDO induced by IFN-γ treatment in iMSCs confirmed by quantitative reverse transcription-PCR (qRT-PCR) (C), immunocytochemistry (D), and flow cytometry©).