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. 2023 Jul 19;24(14):11667. doi: 10.3390/ijms241411667

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibitory effect of NP-2007 on LPS-induced nitric oxide (NO) production and the NF-kB pathway in RAW 264.7 cells. (A) Effects of NP-2007 on RAW 264.7 cell viability. (B) NO production was determined in supernatants from RAW 264.7 cells. (C) Protein expression of iNOS, COX-2, and NF-kB signaling pathways were determined via Western blotting. SDS-PAGE effectively processed equivalent amounts of total proteins. (D) Quantitative analysis of blots. The densitometry results are represented as the relative protein band densities normalized to the β-actin level. All values represent the mean ± SD. Data were examined using Duncan’s multiple comparison test. *** p < 0.001 vs. control group; ### p < 0.001 vs. LPS group.