Skip to main content
. 2023 Jul 18;19(7):e1011233. doi: 10.1371/journal.ppat.1011233

Fig 3. K. pneumoniae serum replication requires purine synthesis and is maximized by endogenous vitamin B6 biosynthesis.

Fig 3

K. pneumoniae strains were grown in M9 salts supplemented with (A) 10% mouse serum or 20% human serum that was either (B) active or (C) heat inactivated. K. pneumoniae strains carrying the empty vector pACYC (ev) or pACYC expressing (D,E) purD (purD+pACYCpurD) or (F) pdxA (ΔpdxA+pACYCpdxA) were grown in 10% mouse serum (D, F). Chemical complementation for purD was measured by supplementation of 1mM purines prior to growth (E). For all, the OD600 was measured every 15 minutes for 12 hours. Differences in growth compared to KPPR1 or KPPR1ev were detected by area under the curve using a one-way ANOVA with Dunnett’s multiple comparison for each strain compared to wild-type; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. For each group, n = 3 in independent trials.