Figure 6.

The transcriptional profiles of mitochondrial functionality markers are different after acute and repeated psychophysical stress of the entire organism in the whole adrenal gland, adrenal cortex, and medulla tissues. Whole adrenal gland (A) tissue as well as adrenal cortex (B) and medulla (C) tissues were isolated from undisturbed rats, as well as acutely (1 × IMO) and repeatedly (2 × IMO and 10 × IMO) stressed rats. Tissue was further used for RNA isolation followed by an analysis of the transcriptional profile of the main markers of mitochondrial functionality. Whole adrenal gland tissue was used for PRKAc and AMPKα1/2 protein expression analysis. The representative blots are shown as panels. Data from scanning densitometry were normalized to ACTIN (endogenous control). Values are shown as bars above the photos of blots. The data bars represent mean ± SEM values of two independent, in vivo experiments (number in brackets above the bars represents number of analyzed animals). Statistical significance was set at level of p < 0.05: * vs. control group and # vs. 1 × IMO group.