Figure 5.

Effect of IPA on activation of NF-κB in RANK-induced RAW 264.7 cells. (A) Protein expression was assessed using Western blot analysis. (B) The activation of p65 was observed using IF staining. These data were observed with an anti-NFATc1 and Alexa Fluor 488 goat anti-mouse antibody by LSM700 Zeiss confocal laser scanning microscope (scale bar: 20 μm). All results were expressed as the mean ± SD (n = 3) of triplicate experiments; ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared with the control group (without RANKL and IPA treatment). * p < 0.05 and **** p < 0.0001 compared with the RANKL-stimulated group. Statistical analysis was calculated using one-way ANOVA.