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Hemolysis assay. Erythrocytes were incubated in the presence of coumarins, and the absorbance of released hemoglobin was measured at 540 nm. Triton X-100 and PBS were used as controls of 100% and 0% hemolysis, respectively. DMSO and acetonitrile were also used as controls. Data are expressed as mean ± standard deviation of three independent experiments. PBS: phosphate-buffered saline. DMSO: dimethyl sulfoxide.
