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. 2023 Jul 28;9(30):eadg7480. doi: 10.1126/sciadv.adg7480

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Fig. 2. — (A) Cryo-EM map of the CBF1:CCAN complex bound to a 30 bp DNA segment of C0N3 containing CDEI, at 3.4 Å. (B) Two subunits of CBF1 interact through their basic α helices with the major groove of the C0N3 DNA at the entry of the CENP-LN-HIK^Head-TW channel . Inset: CENP-LN, CENP-I^Head, and CENP-TW form a basic, closed chamber that topologically entraps DNA. (C) Comparison of the S. cerevisiae and human CCAN^Topo-CENP-A^Nuc (42) modules. S. cerevisiae CCAN topologically entraps the unwrapped C0N3 DNA, 5′ of CENP-A^Nuc, in its basic CBF1:CENP-LN-HIK^Head-TW channel. Human CCAN entraps α-satellite linker DNA in its CENP-LN-HIK^Head-TWSX channel, wrapping the DNA around the CENP-TW histone fold domains.