FIG. 3.
Mapping of 3CLpro cleavage sites. (A) trans-cleavage assays of pp1a/pp1ab amino acids 3490 to 3824. (B) trans-cleavage assays of pp1a/pp1ab amino acids 3630 to 4068. In vitro-translated substrate polypeptides were incubated with buffer (lanes 1, 3, and 5 in panel A and lanes 1, 3, 5, and 7 in panel B) or buffer containing recombinant 3CLpro (lanes 2, 4, and 6 in panel A and lanes 2, 4, 6, and 8 in panel B) for 45 min at 30°C, separated by SDS–17.4% polyacrylamide gel electrophoresis, and analyzed by autoradiography. Sizes of molecular mass markers (lane M) (CFA 626 and 645; Amersham Pharmacia Biotech) are given, and the major cleavage products are indicated.